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Groups of ten size-matched islets were incubated at 37 °C in HEPES-buffered Krebs-Ringer bicarbonate buffer (HKRB) composed of (in mM) 4.7 KCl, 1.2 KH 2 PO 4 , 129 NaCl, 5 NaHCO 3 , 1.2 MgSO 4 , 1.8 CaCl 2 , and 10 HEPES, with pH adjusted at 7.4 using NaOH supplemented with 2.8 mM glucose, followed by test incubation for 1 h with 2.8 or 11.2 mM glucose in the absence or presence of d-allulose (20 mM) or d-mannitol (20 mM)